In vivo evaluation of drug delivery system for treatment of osteomyelitis associated with bone repair

Reference	Presenter	Authors (Institution)	Abstract
02-089	Karen Cristina Kai	Kai, K.C.(Universidade Federal do ABC); de Araújo, D.R.(Universidade Federal do ABC); Cunha, M.R.(Faculdade de Medicina de Jundiaí); Marchi, J.(Federal University of Abc);	Osteomyelitis is a bone infection common in several traumas and results in loss of bone tissue. The conventional treatment is the debridement of infected bone and adjacent soft tissues, associated with administration of antibiotics by systemic route. This work proposes the in vivo evaluation of the abitity of an injectable system based on poloxamer 407 (PL407) incorporated with Mg/Zn-doped ?-tricalcium phosphate (?-TCP) and antibiotic teicoplanin (TC) to regenerate tibial defects and to treat osteomyelitis. ?-TCP was chosen as component of the system for providing bone tissue repair, due its osteoconduction and biocompatibility properties. In a previous work, we have shown that the ?-TCP doped with Mg and Zn improves the thermal stability of the ceramic and increase the bone tissue repair. The matrix of the system was PL407, a thermorreversible, biocompatible and biodegradable hydrogel, which undergoes gelation in situ at 37°C. The in vivo induction of osteomyelitis was performed by inoculation of Staphylococcus aureus in the Wistar rats (Rattus norvegicus, n ? 6) tibia. After 3 weeks of bacteria inoculation in the bone defect, the osteomyelitis was evaluated by radiography. After debridement and washing with saline solution of infection site, the animals were divided into 7 treatment groups: no treatment (control group), teicoplanin by systemic via (14 mg/kg/day), PL407+TC, PL407+TCP, PL407+TCP+TC, PL407+MgZnTCP and PL407+MgZnTCP+TC. After 4 weeks of treatment, the rats were euthanized, the tibia was surgically removed and evaluated by microbiology, radiography and histology. The ?-TCP into the system was suitable to stimulate local osteogenesis in bone tissue defects. The presence of teicoplanin in the drug delivery system decreased the progression of osteomyelitis in rats. The overall results indicated that PL407 based systems incorporated with TCP and teicoplanin are promising for the osteomyelitis local treatment associated with bone tissue repair.
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