Synthesis and Application on metal substrates of ML33 functionalized with Silver Nanoparticles to evaluate antibacterial activity
| Reference | Presenter | Authors (Institution) | Abstract |
|---|---|---|---|
| 18-022 | Suellen Battiston | Battiston, S.(Universidade Federal de Santa Catarina); Bezerra, A.V.(Universidade Federal de Santa Catarina); Ribeiro, L.F.(Federal University of Santa Catarina); Reis, E.M.(Federal University of Santa Catarina); Bernard, S.(University of Limoges); MACHADO, R.A.(Universidade Federal de Santa Catarina); | In
this work, a commercial oligosilazane (ML33) was functionalized with silver
nanoparticles (nAg) to be used as an antibacterial coating applied on a
metallic substrate. While the nAgare well-known antibacterial agent, the ML33
Si-H group provide an excellent adhesion of the coating on the substrate. The
synthesis was performed in solution using DMF as the solvent and AgNO3 as the
nAg precursor. Both, DMF and the ML33 act as a reduction agent of the AgNO3,
which allowed a one-pot synthesis route. Three synthesis temperatures (50, 70,
100°C) were tested and the resulting nAg were characterized by UV spectroscopy
and TEM analysis. At synthesis temperature of 70°C, the
highest concentration of nAg was formed with particles sized in the range of
8-12 nm. Therefore, this sample was selected to be applied on a metallic
substrate by the dip-coating technique, and the applied material was
characterized by MEV-FEG analysis. The metallic substrates after the
application had their biocidal activity tested by the colony counting method.
The bacterial strains were inoculated and separated into two fractions to evaluate
the selected sample and the reference (the metallic substrate coated with pure
ML33). The samples were let in contact with the bacterias for 1, 2, 4, and 6
hours and the biocidal activity was evaluated by the colonies counting after 24
hours. The result revealed that only in the coating containing nAg the colonies
growth for both Gram-positive and Gram-negative bacteria was inhibited,
revealing the potential use of this new material as an antimicrobial coating
for metallic substrates.<!--[if gte mso 9]> |
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