Study of obtaining hydroxyapatite-collagen based biocomposite

Reference	Presenter	Authors (Institution)	Abstract
02-032	Christiane Ribeiro	Ribal, L.F.(Federal University of ABC); Ribeiro, C.(Universidade Federal do ABC (SP), BRAZIL);	The development of materials that act in the process of bone repair has been an alternative for the reconstruction of bone defects, resulting from traumatic injuries, pathological or degenerative diseases. Inspired by the natural composites, recent studies have shown that biocomposites based synthetic hydroxyapatite (HA) and collagen is a success strategy to bone reconstruction/regeneration due to its high biocompatibility and excellent bioactivity. The aim of this study was to obtain a biocomposite (HA/collagen) with suitable characteristics for bone repair; for this the HA was synthesized by neutralization method, adding H3PO4 (0.3M) at 8.0 mL.min-1 rate in Ca(OH)2 (0.5M) suspension. The synthesized ceramic phase was annealed at 800oC for 3 hours in oxidative atmosphere and subsequently, the collagen (bovine nature) in the form of powder was incorporated into ceramic powder at a 30 wt.% ratio. This mixture was kept up in digestion in SBF (simulated body fluid) solution in shaker at 37oC for 12 days, being that the SBF solution was renewed every 3 days. This procedure was performed with goal of promoting the carbonated hydroxyapatite phase conversion and possible mineralization of the collagen in order to mimic structurally the bone biocomposite. Finally, the powders were submitted a freeze-dried process in order to preserve the collagen phase, and then physico chemically characterized. The different phases of the biomaterial composite were evaluated before and after of mineralization process in SBF by XRD analysis and FTIR spectroscopy. The results showed the presence of the carbonated apatite after ripening in SBF. The specific surface area of the ceramic phase was measured by gas adsorption method (BET) and the powder morphology was assessed before and after of mineralization by SEM. The results suggest that technique is effective for production composite (HA/collagen) with similar characteristic to the natural bone tissue, allowing a more suitable repair.
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