Synthesis and antiangiogenic properties of ZnWO4 and ?-Ag2WO4 nanoparticles

Reference	Presenter	Authors (Institution)	Abstract
03-008	Carla Júnia Santos	Santos, C.J.(Universidade Federal de Itajubá); Soares, D.F.(Universidade Federal de Itajubá); FILHO, F.M.(Universidade Federal de Itajubá);	Tungstates are technologically important materials and has attracted a great deal of research attention due to its properties, such as optical, magnetic, electric, photocatalytic, antifungal and antibacterial. However, the biological properties of these materials are almost unexplored. Recently, the antiangiogenic properties of silver nanoparticles and gold nanoparticles was demonstrated. In this context, the antiangiogenic properties of other mettalics nanoparticles, such as ZnWO4 and ?-Ag2WO4, will be evaluated. Thus, the aim of this study was to prepare, characterize and evaluate the angiogenic and cytotoxic properties of ZnWO4 and ?-Ag2WO4 nanoparticles (NPs). ZnWO4 and ?-Ag2WO4 NPs, obtained by the hydrothermal microwave (HM) synthesis, were structurally characterized by X-ray diffraction (XRD) and micro-Raman (MR) spectroscopy. The NPs shapes were obtained by the scanning electron microscopy (SEM). The polydispersity index (PI) were estimated by zeta potential (ZP) analysis. The NPs anti-angiogenic potential was investigated by the chorioallantoic membrane model (CAM) from fertilized chicken eggs. And the nanoparticles cytotoxic activity were evaluated through flow cytometry technique. The XRD analysis revealed structural organization at long range, while MR revealed short range order for all samples. The SEM analyzes suggest that the ZnWO4 NPs have spherical shape and ?-Ag2WO4 NPs have rod-like shape, in nanometric scale. The PI presented by ZnWO4 and ?-Ag2WO4 NPs was 0.58 and 0.18, while ZP was -17.5 and -26.3mV, respectively. The mean percentage of blood vessels remaining in the CAM, after different concentrations application of NPs, were significantly lower than the negative control, thus these result demonstrate that tungstates NPs inhibit angiogenesis in vivo. These NPs does not presented cytotoxic activity against HEK cells. References: Chemical Physics Letters. 2017, 674, 125.; Biomaterials. 2009, 30, 6341.; Angiogenesis. 2011, 14, 409.
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