MS3T – A novel multifunctional platform for health management of HLB affected citrus trees

Reference	Presenter	Authors (Institution)	Abstract
05-016	Maria Gabriela Nogueira Campos	Nogueira Campos, M.(Universidade Federal de Alfenas - UNIFAL - Campus de Poços de Caldas); Tetard, L.(University of Central Florida); Woo Hyoung, L.(University of Central Florida); Johnson, E.(University of Florida); Petridis, L.(University of Tennessee); Labbe, N.(University of Tennessee); Rajasekaran, P.(University of Central Florida); Soliman, M.(University of Central Florida); Chumbimuni Torres, K.(University of Central Florida); Santra, S.(University of Central Florida);	Huanglongbing (HLB; syn citrus greening) disease is an emerging threat for citrus production worldwide. It is caused by the phloem-restricted bacterium, Candidatus Liberibacter asisticus (CLas) and vectored by the Asian Citrus Psyllid (ACP). Disease control is challenging due to high ACP population and lack of suitable systemic bactericides. A multifunctional HLB management tool with ACP control and bactericidal attributes is urgently needed. To meet this need, we have developed a novel multifunctional surface/sub-surface/systemic therapeutic (MS3T) technology. MS3T is formulated with kaolin clay (aluminosilicate; a natural ACP repellent material), which also serves as a delivery system for two potent antimicrobial actives, Quaternary Ammonium Compound (Quat) and Zinc. Quat phytotoxicity is drastically minimized in MS3T through their interaction with clay material (Fixed-Quat). Antimicrobial Zn is in the form of a chelate with urea that serves as a chelator/penetrant. Metal chelates are usually phytotoxic. Nevertheless, phytotoxicity of Zn chelate in MS3T formulation is reduced due to the presence of clay barrier. It is hypothesized that MS3T formulation will serve as a broad-spectrum bactericides/fungicides through combined modes of action. Fixed Quat is surface-restricted, designed for controlling several bacterial and fungal diseases including citrus canker, menalose and scab, while the Zn-chelate is designed as a systemic bactericide for controlling HLB. Metallic Zn uptake by plants was investigated on tomato and citrus using Atomic Absorption Spectroscopy and X-ray Fluorescence Spectroscopy. Metallic Zn concentration on treated plants was dose-dependent, suggesting Zn uptake after MS3T foliar spray. Therefore, clay is an efficient delivery system for Zn-chelate. Also, in vitro studies suggest that MS3T is safe for field application. Field trials have begun in Florida to confirm MS3T efficacy against HLB which might be available prior to this presentation.
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